2.2.3 Invited: Novel Cell-based Biosensing with 2D-SPR imager
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- 2.2 Biosensors II (DNA, SPR)
- Author(s)
- H. Shinohara - Graduate School of Science and Engineering, University of Toyama (Japan)
- Pages
- 173 - 174
- DOI
- 10.5162/IMCS2012/2.2.3
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
In this paper, novel cell-based biosensing system with a high resolution of two dimensional surface plasmon resonance (2D-SPR) imager is introduced. Response of a model nerve precursor cell (PC12) which was adhered on an Au chip to agonist stimulation was observed by the reflection intensity change in the individual cell regions with the 2D-SPR imager at real-time without any probe reagents. It was further demonstrated that the cell response in 2D-SPR imaging to agonist stimulation might be based on intracellular recruitment of signal transduction proteins such as PKC to cell membrane. These results suggested future wide applications of label-free cell-based biosensing with 2D-SPR imager, such as drug screening, cell state evaluation and so on.