P1.3.6 Parallel Analysis of Fluorescence Detection on Multichannel Capillary Electrophoresis Microchip
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.3 Sensors Based on Optical Techniques
- Author(s)
- Z. Liu, W. Yan, X. Yang, H. Lv, Z. Tang - School of Electronic Science and Technology, Dalian University of Technology (China)
- Pages
- 942 - 945
- DOI
- 10.5162/IMCS2012/P1.3.6
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
A simple and compact parallel analysis of fluorescence detection system with cross-polarization was presented and applied to multi-channel capillary electrophoresis. This device was demonstrated using a array of surface mounted devices light-emitting diode (SMD LED) as the excitation light source and a linear charge coupled device (CCD) as a photodetector. A cost-effective cross-polarization scheme was employed to eliminate almost all of the excitated light. With the combination of polarizer and CCD, the provided system could detect different concentrations of one dye or multiple dyes simultaneously because of the capability of CCD and polarizers responding to the wavelength of visible light. This system has been used for fluorescence detection of capillary electrophoresis microchip with Rhodamine 6G, Rhodamine B and fluorescein and the limit-of-detection were 1µM, 10µM, 100µM respectively. The project opens a door to integrate high-sensitivity microchip with light source and detector for inexpensive and relatively simple fluorescence-based analytical system.