P1.3.11 Novel immune TiO2 photoluminescence biosenors for leucosis detection
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.3 Sensors Based on Optical Techniques
- Author(s)
- R. Viter, V. Smyntyna, A. Tereshchenko, A. Kusevitch, I. Doycho, S. Geveluk, J. Lubchuk, I. Konup - Odessa National I.I. Mechnikov University (Ukraine), N. Starodub, N. Slishik - National University of Life and Environmental Sciences (Ukraine), J. Buk - Kertak Nanotechnology (Czech Republic), J. Duchoslav - Johannes Kepler Universität (JKU) (Austria), A. Ubelis, J. Spigulis - University of Latvia (Latvia)
- Pages
- 959 - 962
- DOI
- 10.5162/IMCS2012/P1.3.11
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
Novel immune photoluminescent biosensor, based on TiO2 nanoparticles, for retroviral leucosis detection has been developed. The photoluminescence spectra were excited by solid state laser with wavelength 355 nm and measured in the range of 370-800 nm. Original photoluminescence spectrum of TiO2 nanoparticles showed wide maximum at 515 nm. The biosensitive layer was formed by immobilization of retroviral leucosis antigens on the surface of TiO2 nanoparticles. Immobilization of antigens on TiO2 surface led to UV-shift of photoluminescence spectrum and increase of PL intensity. The response to different concentrations of retroviral leucosis antibodies has been measured. The decrease of spectrum intensity and IR-shift were observed after antibodies adsorption on biosensor surface. The experimental dependences of maximum shift and intensity changes versus antibodies concentration were obtained.