J2 - Non-invasive Monitoring of Bioreactors Using Atmospheric Pressure Chemical Ionization Mass Spectrometry
- Event
- 11. Dresdner Sensor-Symposium 2013
2013-12-09 - 2013-12-11
Dreikönigskirche - Haus der Kirche Dresden - Chapter
- 9. Nicht invasive Prozessmesstechnik
- Author(s)
- J. Langejuergen, M. Buering, C. Wagner, S. Beutel, T. Scheper, S. Zimmermann - Leibniz University Hannover/D
- Pages
- 399 - 402
- DOI
- 10.5162/11dss2013/J2
- ISBN
- 978-3-9813484-5-3
- Price
- free
Abstract
Generating biomass or biochemically active substances with constant and high quality and quantity requires continuous control of the biochemical environment within the used bioreactor. Thus, sensors or sensor systems that monitor the conditions during the cultivation are required. Since in most bioprocesses it is indispensable to prevent any contamination of the culture medium a continuously working sensor in these applications must either stand the process of sterilization or be non-invasive. In this work we present first results from a non-invasive analysis of the waste gas of a 2 L bioreactor
during the cultivation of a model organism (E. coli strain K12) using a mass spectrometer with atmospheric pressure chemical ionization and a gas chromatographic pre-separation. Based on the used ionization method a detection of volatile organic compounds emitted during the lag-phase or the growth of the bacteria with extremely low detection limits is possible. We repeatedly observed various compounds with an abundance correlated to the growth of the culture which was monitored by further invasive and non-invasive sensors.