BS1.4 - Graphene Hybrids: Functionalized Nanomaterial for Single-step Immunoassay Based on Fluorescence Quenching and Size Separation
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 1
- Author(s)
- A. Shirai, K. Sueyoshi, T. Endo, H. Hisamoto - Osaka Prefecture University, Sakai-shi (Japan)
- Pages
- 75 - 76
- DOI
- 10.5162/IMCS2018/BS1.4
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
This paper reports the preparation of the polyethylene glycol-coated graphene nanoplatelet (PEG-GN) possessing highly dispersive, fluorescence quenching and size separation functions, and its application to "single-step" immunoassay. PEG-GN allowed to separate free fluorescently-labeled antibody and immunocomplex by the difference in size. The former adsorbed on PEG-GN and its fluorescence was quenched, and the latter did not approach the surface of PEG-GN due to the steric hindrance of PEG structure. By mixing PEG-GN and fluorescently-labeled antibody and sample solution containing antigen, the antigen concentration was able to be measured from the fluorescence intensity changes without washing operation. In this study, as a demonstration, the C-reactive protein (CRP) concentration was successfully measured by single-step operation.