BS3.3 - Enzyme-modified carbon-felt based flow-type amperometric glucose biosensor using oxidase/peroxidase-bienzyme system
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 3
- Author(s)
- Y. Hasebe, T. Watanabe - Department of Life Science and Green Chemistry, Saitama Institute of Technology, Fukaya (Japan)
- Pages
- 139 - 140
- DOI
- 10.5162/IMCS2018/BS3.3
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Amperometric flow-injection analysis (FIA)-type glucose biosensor was fabricated using porous carbonfelt modified with glucose oxidase (GOx) and horseradish peroxidase (HRP). Enzyme-immobilization (adsorption) protocol and operational parameters were optimized. The obtained GOx/HRP-CF-based FIA-type glucose biosensor showed the sensitivity (2.07 μA/mM), the limit of detection (0.014 mM), and linear range (0.03 to 3 mM). Interference by ascorbic acid (0.5 mM) could be cancelled by the control of applied potential. The RSD for 63 consecutive injection of 10 mM glucose was 3.71 %. The biosensor was applied for the determination of glucose in various beverages, and the analytical results obtained by the sensor were in fairly good agreement with those by conventional enzyme-based spectrophotometry.