4.2.4 Piezoelectric Olfactory Receptor Biosensor with Aptamer assisted Immobilization Technique
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- 4.2 Biosensors IV (Systems)
- Author(s)
- L. Du, C. Wu, P. Wang - Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University (China), H. Peng, L. Zhao - Department of Biochemistry and Genetics, School of Medicine, Zhejiang University (China)
- Pages
- 348 - 351
- DOI
- 10.5162/IMCS2012/4.2.4
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
This paper presents an olfactory receptor-based piezoelectric biosensor for sensitive odor detection, in which aptamer-assisted olfactory receptor immobilization method is employed for the purpose of performance enhancement. Olfactory receptors (ORs) with a his-tag were employed as the sensitive elements. Anti-his aptamers were immobilized covalently onto the gold surface of piezoelectric quartz crystal microbalance (QCM) electrode to capture his-tagged ORs specifically. By this method, the covalent immobilization as well as the purification of ORs was obtained simultaneously. The results demonstrated that aptamer-assisted OR immobilization technique can improve the performances of the biosensor greatly. Specifically, the detection limit of this OR-based biosensor is as low as 2-3 ppm.