5.1.1 Detection of pathogenic Staphylococcus aureus bacteria by Electrochemical Impedance
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- 5.1 Impedance-based Sensing
- Author(s)
- M. Braiek, A. Maaref - Laboratoire de Physique et Chimie des Interfaces, Faculté des Sciences de Monastir (Tunisia), N. Jaffrezic-Renault - Laboratoire des Sciences Analytiques, UMR CNRS 5180, Université Claude Bernard-Lyon1 (France), K. Bekir Rokbani, B. Mrabet, A. Bakhrouf - Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Faculté de Pharmacie de Monastir (Tunisia)
- Pages
- 418 - 420
- DOI
- 10.5162/IMCS2012/5.1.1
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
The timely detection of pathogens is a subject of great importance. In this work, the objective is to elaborate an immunosensing system for detection and quantification of Staphylococcus aureus. Poyclonal anti-S. aureus are immobilized onto gold electrodes via chemical bond formation between antibody amino groups and a carboxylic acid containing self-assembled molecular monolayer. The evaluation of the developed immunosensor performance was accomplished through the monitoring of the electron-transfer resistance detected by electrochemical impedance spectroscopy in the presence of [Fe(CN)63-]/[Fe(CN)6 4- ] as redox probe. A low detection limit of 10 fcu/ml and a linear range up to 10^7 cfu/ml were obtained.