5.1.3 DNA electrodes for detection of sequence specific nucleic acid-ligand interaction
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- 5.1 Impedance-based Sensing
- Author(s)
- C. Tersch, C. Witte, F. Lisdat - Biosystems Technology, Wildau Technical University of Applied Sciences (Germany), J. Glöckler - Alacris Theranostics GmbH/ MPI for Molecular Genetics (Germany)
- Pages
- 422 - 423
- DOI
- 10.5162/IMCS2012/5.1.3
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
In this study an impedimetric DNA sensor has been used for the detection of protein-DNA interactions. The sensor is based on single-stranded DNA bound to gold chip electrodes. In the presence of ferri/ferrocyanide impedance measurements show an increase in charge transfer resistance Rct after immobilization and hybridization of ssDNA to the sensor surface. This allows hybridization detection in the low nanomolar concentration range. This electrode system is used to follow the sequence specific reaction of the restriction endonuclease BamHI on the surface electrochemically. Furthermore, the sequence specific DNA-binding of the transcription factor NF-kB p50 is found to cause a decrease in Rct resistance in a concentration dependent way.