P1.2.11 An Integrated Electrophoresis Microchip for Label-free Detection Using Surface Plasmon Resonance Image
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.2 Resonant Sensors
- Author(s)
- X. Chen, H. Cai, L. Zhang, H. Li, J. Sun, D. Cui - State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences (China), Y. Chao - Institute of Microbiology, Chinese Academy of Sciences (China)
- Pages
- 920 - 922
- DOI
- 10.5162/IMCS2012/P1.2.11
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
In this work, a novel microfluidic-based electrophoresis method combined with surface plasmon resonance image (SPRI) has been developed for electrokinetic fluid transport and label-free detection. The integrated microfluidic chip was designed and fabricated by MEMS technology. A PDMS layer with double T-type microchannels for electrophoresis was fabricated and then directly bonded on a specialized SPR sensing substrate. In order to avoid the electrochemical corrosion of the metallic SPR surface, a microarray of rectangle gold spots was instead of an intact gold film. In a case, FITC in TBE buffer was been electrically driven and detected in the integrated microchip using our SPRI system under 100V/cm electric field strength. The applied electric field with high conductivity buffers in our integrated microchip is much higher than that reported in the past literatures, which has the greatest potential for various real-time electrophoresis separation and label-free detection in the fields of genetic analysis, protein assay, environmental testing, biological warfare agent detection, drug discovery and so on.