BS5.2 - Development of Rapid Assays for Detection and Identification of Pathogenic Bacteria
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 5 - Biomimetic Approaches
- Author(s)
- S. Li, H. Li - Department of Chemistry, National University of Singapore, Singapore (Singapore)
- Pages
- 209 - 210
- DOI
- 10.5162/IMCS2018/BS5.2
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Bacterial contamination in water and food poses serious hazards worldwide and the rapid detection of pathogenic bacteria is critical to the prevention and identification of problems related to public health and safety. This study focuses on the development of rapid detection assays by using biosensors for rapid, sensitive, specific pathogens detection in water or complex food matrices. Quartz crystal microbalance biosensors with different bio-recognition elements, strategies for improved sensitivity by nanomaterials, sample preparations to eliminate interference were explored. The measurements in all biosensors were reproducible with relative standard deviation (RSD) less than 15%. Non-cultural method for food sample preparation was built to rapidly and efficiently separate most of the interfering particles without sacrificing bacterial recovery. Furthermore, nanomaterials were utilized for improving sensitivity of biosensors. Gold nanoparticles (AuNPs) were synthesized and characterized for QCM signals amplification. Label-free QCM biosensors with different sensitivity and assay time based on self-assembled monolayers (SAMs) have been set up. A detection limit as low as ~102 CFU/mL for E. coli could be achieved when AuNPs were used and total assay time was less than 2 h.