BS5.5 - Highly Sensitive Rapid DNA Sensor Based on Light-induced Acceleration of Molecular Recognition
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- Biosensors 5 - Biomimetic Approaches
- Author(s)
- T. Iida, Y. Nishimura, M. Tamura - Department of Physical Science, Graduate School of Science, Osaka Prefecture University, Osaka (Japan), K. Nishida - Research Institute for Light-induced Acceleration System (RILACS), Osaka (Japan), S. Ito, S. Tokonami - Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Osaka (Japan)
- Pages
- 216 - 217
- DOI
- 10.5162/IMCS2018/BS5.5
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Molecular recognition is a fundamental mechanism for the specific detection in various types of biosensor technology. Here, we theoretically and experimentally demonstrated the "Light-induced acceleration of molecular recognition", and succeeded in the rapid detection of zeptomole-level DNA. The formation of a submillimetre network-like structure was observed at desired position under within several minutes via the laser-induced hybridization of DNA-modified NPs and target DNA exhibiting a broadband optical spectrum. This finding describes a strategy for the remote control of hybridization of extremely small amounts of DNA (1/1000,000 of that without laser irradiation) as a trigger, and for not only rapid bottom-up production of macroscopic assembly with NPs even without PCR amplification, but also rapid and highly sensitive genetic screening without fluorescent labeling.