A5 - Development of an Orthogonal Dual Delay Line Biosensor with AcousticMixing for Enhanced Sensitivity on a ST-Quartz Substrate
- Event
- 20th Jubilee Winter Workshop
on Acoustoelectronics
2024-02-24 - 2024-02-27
Szczyrk, Beskidy Mountains (Poland) - Chapter
- Acoustoelectronics
- Author(s)
- Y. Huang, V. Bhethanabotla - University of South Florida, Tampa (USA)
- Pages
- 14 - 15
- DOI
- 10.5162/20WWonAE/A5
- ISBN
- 978-3-910600-05-8
- Price
- free
Abstract
This study discussed the development of an orthogonal dual-delay line biosensor fabricated on an STQuartz substrate, which combines of Rayleigh wave SAW propagation in orthogonal to the SH-SAWs was used to enhance biosensing sensitivity and binding kinetics through Rayleigh wave streaming. The SH-SAW waveguides were optimized and demonstrated reduced insertion loss and maximized acoustic energy confinement to improve sensor’s performance. The implementation of a dual-line configuration also provides a reference sensing line that minimized thermal variation and environmental noises presented in sensing data. Binding experiments between Protein G and rabbit anti-mouse IgG showed that SAW mixing can lead to a stronger binding affinity compared to the nonmixing case. These results highlight the efficacy of Rayleigh wave mixing in reducing assay time, improving binding efficiency, and enhancing sensor sensitivity, and demonstrate a low-cost sensor with the potential to facilitate experiments where multiple affinity bindings need to be performed at low concentrations.