P2.1.12 Label Free Biochemical Determinations Based on the Contrast Monitoring of Periodic Patterns
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P2.1 Biosensors
- Author(s)
- I. Archonta, A. Salapatas, K. Misiakos - Microelectronics Institute, NCSRD Demokritos (Greece)
- Pages
- 1359 - 1361
- DOI
- 10.5162/IMCS2012/P2.1.12
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
A method is presented for label-free detection of protein-protein binding in real time. It is based on the protein induced contrast modulation of the microscope image of an oxidized and patterned silicon substrate. Interferometric effects make the reflection coefficient of the solution-protein-oxide-silicon stack a function of the oxide-protein combined thickness. As a consequence, the contrast of the pattern changes during the experiment and the magnitude of the change is a measure of the progress of the biomolecular reaction. A model bioanalytical assay demonstrates the potential of the method.