P2.1.13 Towards the detection of static ATP levels above primary PTPRζ-osteoblastic cells and their knock-out mutants by ATP microbiosensors
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P2.1 Biosensors
- Author(s)
- C. Steinbach, E. Hecht, B. Mizaikoff, C. Kranz - Institute of Analytical and Bioanalytical Chemistry, University of Ulm, S. Dobler, A. Liedert, A. Ignatius - Institute of Orthopaedic Research and Biomechanics, University of Ulm (Germany)
- Pages
- 1362 - 1363
- DOI
- 10.5162/IMCS2012/P2.1.13
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
Adenosine-5`-triphosphate (ATP) holds a significant role as omnipresent energy source and as autocrine and paracrine signaling molecule in many cells such as lung cells and bone cells. ATP is considered to be involved in the mechanical stress response of bone cells such as bone-resorbing osteoblast cells or receptor-proteine-tyrosinephosphatase-zeta (PTPR_) - osteoblastic cells, which are involved in bone formation, bone regeneration and the control of bone volume. ATP release stimulates the proliferation of these P2 - receptor cell types. The “deficient” knock-out mutant behaves different in proliferation and differentiation and thus the ATP release above these cells is expected to be altered. A localized detection of ATP at the cellular level is therefore of significant importance. Using amperometric ATP microbiosensors with diameters ranging from 10 - 50 μm enables localized ATP measurements above wild type and knock-out cells.