C4.1 - Model-Based Design and Optimization of Microfluidic Systems for Gentle Cellular Perfusion
- Event
- AMA Conferences 2017
2017-05-30 - 2017-06-01
Nürnberg, Germany - Band
- Proceedings Sensor 2017
- Chapter
- C4 - Modeling and Simulation II
- Author(s)
- M. Gusenbauer, G. Mazza, M. Brandl, T. Schrefl - Donau-Universität Krems (Austria), R. Tothova, I. Jancigova, I. Cimrak - University of Zilina (Slovakia)
- Pages
- 348 - 351
- DOI
- 10.5162/sensor2017/C4.1
- ISBN
- 978-3-9816876-4-4
- Price
- free
Abstract
Cell sensitive applications, like dynamic cell culturing, require the physiological perfusion of cell samples to the desired analysis slide. Stress occurring inside the flow chambers, connectors and pumping circuits can cause inflammatory reactions in blood cells or even cell-damage. Quantification of the mechanical stimuli with computer simulations enables the optimization of the system geometry, thus the minimization of the blood damage or activation. We use an automated procedure to calculate
blood damage, regarding whole blood both as a continuum as well as a cell suspension. The obtained cell damage information feeds a mathematical optimization algorithm. The setup can be used to analyze and optimize arbitrary microfluidic systems for cell biological applications. Recent results of an optimized inlet of a flow chamber and a gentle pumping device are demonstrated.