P2BM.9 - A Sensor Made from A Paste Electrode of Graphite Particles Grafted with Molecularly Imprinted Polymer
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-2 - Biomimetic Sensors
- Author(s)
- Y. Yoshimi, R. Yamaguchi, M. Seki - Shibaura Institute of Technology, Tokyo (Japan)
- Pages
- 701 - 702
- DOI
- 10.5162/IMCS2018/P2BM.9
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
A real-time heparin monitor could be used to optimize the dosage of heparin during extracorporeal circulation procedures. This report describes the development of a graphite-paste (GP) electrode with molecularly imprinted polymer (MIP) grafted onto it. Heparin-imprinted poly (methacryloxyethyltriammonium chloride -co- acrylamide -co- methylenebisacrylamide) was grafted directly onto graphite particles. The grafted particles were thoroughly mixed with oil to fabricate the MIP-GP electrode. Traditional cyclic voltammetry was performed with the electrode in physiological saline or bovine whole blood containing 5 mM ferrocyanide and 0-8 units/mL heparin. The current intensity increased with heparin concentration, due to expansion of the effective surface area resulting from heparin-promoted mobility of the oil in the MIP-GP electrode.The MIP-GP electrode, which operated through a new heparin-sensing mechanism, is an excellent candidate for application as a disposable sensor to monitor heparin levels in blood.