P1.1.7 Fast detection of pathogenic bacteria by using different sensor techniques
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.1 Biosensors
- Author(s)
- K. Kleo, C. Nietzold, F. Lisdat - University of Applied Sciences Wildau, Biosystems Technology, R. Grunow - Centre for Biological Security (ZBS2)
- Pages
- 834 - 836
- DOI
- 10.5162/IMCS2012/P1.1.7
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
Francisella tularensis are gram-negative bacteria capable of infecting mammals. This high pathogenic species is classified as a potential bioterroristic agent by the Centers for Disease Control and Prevention, Atlanta / USA. Therefore, a renewed interest in studying F.tularensis bacteria can be observed. This is strongly focused on developments of fast, cheap, robust, sensitive and specific detection methods. With this work we want to present alternative sensor systems for these pathogenic bacteria. Here we demonstrate two different immunological detection systems for F.tularensis. First technique is based on quartz crystal microbalance (QCM) which uses sensor chips modified by a specific antibody.The QCM technique is combined with a microfluidic flow system and allows the online detection of binding events between antibody and whole bacteria. The second system is based on the aggregation of antibody-functionalized gold nanoparticles and bacteria. This is monitored by a change of particle diameter using dynamic light scattering and of absorption peak shift by UV/Vis spectroscopy. A detection limit of 10^3 CFU/ml can be obtained.