P1.1.23 Cerium oxide ISFET based immune biosensor for control of bacterial contamination
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.1 Biosensors
- Author(s)
- N. Starodub, J. Ogorodnijchuk - National University of Life and Environmental Sciences of Ukraine (Ukraine)
- Pages
- 880 - 883
- DOI
- 10.5162/IMCS2012/P1.1.23
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
A new cerium oxide ISFET based immune biosensor for the determination of Salmonella in solution was developed. It was chosen "sandwich" algorithm of analysis when one part of specific antibodies was immobilized on the transducer surface and other one labeled by the horse radish peroxidase (HRP) was added to analyzed solution. Overall time of analysis was about 15-20 min. The maximal sensitivity of S. typhimurium determination in model solutions was on the level of 2-3 cells ml^-1 with the linearity of the immune biosensor response up to 5x10^5 cells ml^-1.