P1.1.13 Thin Films of Substituted Polyanilines: Interactions with Biomolecular Systems
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.1 Biosensors
- Author(s)
- D. Sarauli, F. Lisdat - Biosystems Technology, Wildau University of Applied Sciences (Germany), C. Xu - UP Transfer GmbH (Germany), B. Dietzel, B. Schulz - University of Potsdam, Institute for Physics and Astronomy (Germany), K. Stiba, S. Leimkühler - University of Potsdam, Institute for Biochemistry and Biology, Department of Molecular Enzymology (Germany)
- Pages
- 851 - 855
- DOI
- 10.5162/IMCS2012/P1.1.13
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
Differently substituted polyaniline copolymes are shown to provide interfaces for the communication with biosystems. The various substitutions of the aromatic ring are influencing the conductivity properties of the polymer immobilized on electrode surfaces, and lead to a modified interaction with proteins and/or enzyme molecules. It is shown that an analytical signal chain starting from the substrate sulfite in solution via the enzyme sulfite oxidase and the redox protein cyt c to the polymer can be constructed. The electron transfer is also shown without the use of an electric field. The enzymatic system induces redox switching between oxidized and reduced states of polymer, which can be visualized not only in solution, but also with a thin polymer film on electrodes.