P1.1.18 Real-time monitoring of cell response to drug stimulation by 2D-SPR Sensor: An approach to study neuronal differentiation
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.1 Biosensors
- Author(s)
- T. Mir, H. Shinohara - Course of Life Information and System Science, Graduate School of Innovative Life Science for Education, University of Toyama (Japan)
- Pages
- 863 - 865
- DOI
- 10.5162/IMCS2012/P1.1.18
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
There is a growing demand for the development of new bio-analytical technique that is capable of monitoring neuronal differentiation non-invasively, in real-time and without any fluorescent or radioactive probes. To fulfill these promises, efficient and easy protocols to monitor neuronal differentiation have to be developed. In this study, we demonstrated a rapid and label-free detection method to monitor neuronal differentiation by high-resolution 2Dsurface plasmon resonance sensing technology with a CCD camera to analyzing refractive index change of PC12 cells. We have successfully monitored the intracellular translocation of PKC in PC12 cells by 2D-SPR and found that the cells treated with differentiation factor, nerve growth factor (NGF) showed a remarkable enhancement of SPR response to muscarine and carbacol stimulation. Our results suggested that 2D-SPR imager might be applicable to monitor neuronal differentiation and to study the expression state of the specific receptors in living state.