P1.1.24 Efficiency of immune biosensor based on total internal reflection ellipsometry at the determination of Salmonella
- Event
- 14th International Meeting on Chemical Sensors - IMCS 2012
2012-05-20 - 2012-05-23
Nürnberg/Nuremberg, Germany - Chapter
- P1.1 Biosensors
- Author(s)
- N. Starodub, J. Ogorodnijchuk - National University of Life and Environmental Sciences of Ukraine (Ukraine)
- Pages
- 884 - 887
- DOI
- 10.5162/IMCS2012/P1.1.24
- ISBN
- 978-3-9813484-2-2
- Price
- free
Abstract
It was investigated the efficiency of the immune biosensor based on the total internal reflection ellipsometry (TIRE) comparing with surface plasmon resonance (SPR) based biosensors for determination of such bacteria as Salmonella in model solutions. It has been defined that in case with Spreeta sensitivity was on the level 10^3 - 10^7 cells/ml. Another biosensor based on the SPR has shown the sensitivity within 10^1 - 10^6 cells/ml. The maximal level of sensitivity was obtained using TIRE based device and it was on the level of several cells in 10 ml (up to the fact that less than 5 cells). The optimal time of the sample incubation with the transducer surface was about 15 min Also it was evaluated possibility of practical application of the above mention instrumental approaches.