P1BS.1 - A Novel Electrochemical Enzyme Biosensor for Detection of 17β-Estradiol by Mediated Electron-transfer System.
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- A. Wang, Y. Ding, L. Li, Y. He, C. Wang, Q. Mei, D. Duan - Department of Chemistry, Shanghai University, Baoshan District, Shanghai (China)
- Pages
- 476 - 477
- DOI
- 10.5162/IMCS2018/P1BS.1
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
In this work, an extremely sensitive enzyme sensor for detection of 17 -estradiol based on electropolymerized Llysine molecules on a glassy carbon electrode (GCE) modified with critic acid@graphene (CA-GR) and cross-linked with laccase enzyme has been developed in this work. The morphology of the enzyme modified electrode was characterized by TEM, SEM and FTIR. The electrochemical detection of 17β-Estradiol was successfully conducted by mediated electron-transfer system and detection limit of 17β-Estradiol was as low as 0.13 pM. The human urine samples analysis confirmed the application of this enzyme sensor to quantitative analysis of ultra-trace hormone.