P1BS.10 - A Novel Design of Cell-based Biosensors for Detecting Cell Acidification
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- P. Wang, T. Liang, Q. Wu, J. Tu, Y. Gan, Z. Qin, H. Wan - Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang (China)
- Pages
- 493 - 494
- DOI
- 10.5162/IMCS2018/P1BS.10
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Cell metabolism is a common biological mechanism in living cells. Acidification plays an important role in cell metabolism, thus the extracellular pH change is used to indicate the vitality of cells. For extracellular detection of cell metabolic substances, light addressable potentiometric sensor (LAPS) has many advantages, such as high sensitivity, easy encapsulation, and perfect to make microchambers for cell experiments. LAPS is a spatially resolved biochemical sensor based on fieldeffect. Characteristic test experiments and cell metabolism experiments were carried out to determine the performance of LAPS by monitoring the pH change of human colon adenocarcinoma cells Caco-2. The pH sensitivity is 53.69mV/pH, the pH of Caco-2 cells changes from 6.5 to 5.2 and the rate of pH change decreases after 30min. The results showed that the system performed well in real-time detection of cell acidification.