P1BS.2 - Electrochemical Paper-based DNA Sensor for Human Papillomavirus Detection
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- P. Teengam, T. Vilaivan, O. Chailapakul - Chulalongkorn University, Pathumwan, Bangkok (Thailand), W. Siangproh - Srinakharinwirot University, Bangkok (Thailand), A. Tuantranont - National Electronics and Computer Technology, Pathumthani (Thailand), C. Henry - Colorado State University, Fort Collins (USA)
- Pages
- 478 - 478
- DOI
- 10.5162/IMCS2018/P1BS.2
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
A novel paper-based electrochemical biosensor using an anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe (AQ-PNA) and graphene-polyaniline (G-PANI) modified electrode was developed for human papillomavirus (HPV) detection. An inkjet printing technique was employed to prepare the paper-based G-PANI-modified working electrode. The AQ-PNA probe baring a negatively charged amino acid at the N-terminus was immobilized onto the electrode surface through electrostatic attraction. Electrochemical impedance spectroscopy (EIS) was used to verify the AQ-PNA immobilization. The paper-based electrochemical DNA biosensor was used to detect an oligonucleotide target with a sequence corresponding to HPV type 16 by measuring the electrochemical signal response of the AQ label using square-wave voltammetry before and after hybridization. After the addition of target DNA, the current signal significantly decreased. This phenomenon is explained by the rigidity of PNA-DNA duplexes, which obstructs the accessibility of electron transfer from the AQ label to the electrode surface. Under optimal conditions, the linearity in the range of 10-200 nM with the detection limit of 2.3 nM was obtained. The performance of this biosensor on real DNA samples was tested with the detection of PCR-amplified DNA samples from the SiHa cell line. The proposed method employs an inexpensive and disposable device and is promising for the screening and monitoring of the amount of HPV-DNA type 16 to identify the primary stages of cervical cancer.