2025 SMSI Bannerklein

P1BS.12 - The Specificity and Sensitivity of PRRSV Aptamer using Quartz Crystal Microbalance (QCM)

Event
17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria
Chapter
P-1 - Biosensors
Author(s)
K. Choowongkumon - Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok (Thailand), C. Kuitio - Genetic Engineering, Faculty of Graduate School, Kasetsart University, Bangkok (Thailand), P. Lieberzeit - Department of Physical Chemistry, University of Vienna, Vienna (Austria)
Pages
497 - 498
DOI
10.5162/IMCS2018/P1BS.12
ISBN
978-3-9816876-9-9
Price
free

Abstract

The procine reproductive and respiratory syndrome (PRRS) cause by RNA virus, that is the important disases make economic lose in swine industry. The best for prevention from virus is screening swine befor come to the fram. Aptamer is a short ss-DNA that can bind with small molecule, cell, bacteria and virus, so the aptamer colud be develop to biosensor for screening the infection of procine reproductive and respiratory syndrome virus (PRRSV) in swine. In this study, want to test the specificity and sensitivity of PRRSV aptamer (7R) by quarzt crystal microbalance (QCM). The result from QCM show 7R aptamer could be bind with PRRSV around 10 10 virus particles and 7R aptamer cloud not bind with Pseudorabies virus (PRV) and Classical swine fever vius (CSFV).

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