P1BS.8 - Enzyme Biosensor Based on Tobacco Mosaic Virus-Modified Field-Effect Structures
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- M. Jablonski, A. Poghossian, D. Molinnus, M. Schöning - Institute of Nano- and Biotechnologies, FH Aachen, Campus Jülich, Juelich (Germany), C. Koch, C. Wege - Institute of Biomaterials and Biomolecular Systems, Universität Stuttgart, Stuttgart (Germany)
- Pages
- 489 - 490
- DOI
- 10.5162/IMCS2018/P1BS.8
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
A novel approach for the development of enzyme biosensors based on field-effect structures modified with tobacco mosaic virus (TMV) as enzyme nanocarrier is presented. TMV nanoparticles serve as scaffolds for precisely positioned high-density enzyme immobilization with retained enzyme activity. To prove this new concept, a capacitive penicillin biosensor based on an electrolyte-insulatorsemiconductor structure modified with penicillinase-functionalized TMV nanotubes was realized as model system. The fabricated sensor has been electrochemically characterized by capacitancevoltage and constant-capacitance measurements. The sensor surface morphology with TMV nanotubes was physically investigated by scanning electron microscopy and atomic force microscopy. The results of this study demonstrate the promising potential of virus particles as universal scaffolds for the immobilization of biomolecules on sensor surfaces and for the development of highly sensitive enzyme biosensors.