P1BS.6 - Cell Culture Growth Monitoring using Disposable Electrochemical Enzymatic Sensors
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- P. Fanjul Bolado, M. Neves, M. González García, D. Hernández Santos - DropSens, S.L. Edificio CEEI, Llanera, Asturias (Spain)
- Pages
- 485 - 486
- DOI
- 10.5162/IMCS2018/P1BS.6
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Determination of cell growth and viability is crucial for bioprocesses monitoring. Traditionally, the control of the cell culture growth is performed by cell counting, which can be a time-consuming procedure that requires an expert user. Thus, in order to take the cell culture control to the next level, electrochemical enzymatic sensors for the in-situ measurement of substrates and metabolites of interest in cell cultures were developed. Two of the most commonly monitored parameters are glucose, as the major carbon and energy source, and lactate, as a metabolic product. Therefore, two amperometric enzymatic sensors specific towards this key analytes were developed. Glucose and lactate sensors were based on screen-printed miniaturized technology which allowed collecting samples from the media in order of microliters, without disturbing the cell culture. The proposed glucose and lactate sensors were able to distinguish differences in glucose and lactate levels in cell media 24 h after the cells have been seeded. Due to the quantitative establishment of the uptake of glucose and production of lactate it was possible to effectively control the growth process stages. Aspects such as the limits of detection, reproducibility and repeatability were studied and the figures of merit were obtained. The developed sensors revealed to be robust, highly stable, user-friendly and time-saving, demonstrating the possibility of being used as a cell culture monitoring trustful tool.