P1BS.7 - An impedimetric immunosensor based on magnetic graphene nanoribbons modified by boronic acid for sensitive label-free detection of lymphoma cancer cells
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- P. Hashemi, A. Afkhami - Faculty of Chemistry, Bu-Ali Sina University, Hamedan (Iran), H. Bagheri - Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran (Iran)
- Pages
- 487 - 488
- DOI
- 10.5162/IMCS2018/P1BS.7
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Cancer, as the second most common cause of death in the world, present a major and unmet challenge to healthcare globally. It has been reported that very sensitive monitoring of cancer cells could provide an easier and more effective way to monitor the progression of the disease. Thus, there is an immediate need for new detection and identification methods. The integral membrane protein CD20 was first identified as a marker for B cells over 20 years ago [4]. In this work, for label-free and direct detection of non-Hodgkin's lymphoma (NHL) in early stage, an impedimetric immunosensor based on a magnetic graphene nanoribbons (MGNRs) modified by boronic acid, anti-body and BSA (MGNRs/BA/Ab/BSA) composite was fabricated and evaluated. The physicochemical and analytical measurements were performed to examine the properties of nanocomposite and to determine the influence upon immunosensor performance to CD20 antigen detection. Impedimetric measurements showed significant changes in charge transfer resistance upon binding of cancer cells. The measurements were highly target specific, linear with logarithmic cell concentrations in PBS and human blood sample across a 100 cells/mL and 1000000 cells/mL range and associated with a detection limit of 38 cells/mL.