OT6.15 - Microfluidic Rolling Circle Amplification Device for Pathogen Detection in Food Products
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Lectures
- Chapter
- OT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- R. R. Rosa, V. Chu, J. P. Conde - Instituto de Engenharia de Sistemas e Computadores (INESC-MN), Lisbon (Portugal), A. M. Fortes - Universidade de Lisboa (BioISI ), Lisbon (Portugal)
- Pages
- 153 - 154
- DOI
- 10.5162/EUROSENSORSXXXVI/OT6.15
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
Due to climate change, infections in plants have become increasingly frequent and damaging. For this reason, methods to provide early detection of infection are crucial to allow for timely treatment to prevent the spread of the infection and to safeguard the crop. In this work, we have developed a microfluidic device to amplify DNA through rolling circle amplification using microbeads as a solid platform to immobilize the DNA. We demonstrate the application of this device towards the detection of Botrytis cinerea, a pathogen that has a major impact on several crops.