OT6.217 - 3D Modelling of droplet formation in two-phase microfluidics for single-cell manipulation
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Lectures
- Chapter
- OT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- Z. Szomor, E. L. Tóth, P. Fürjes - HUN-REN Centre for Energy Research, Budapest (Hungary)
- Pages
- 183 - 184
- DOI
- 10.5162/EUROSENSORSXXXVI/OT6.217
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
In this study, the evolving hydrodynamic phenomena were modelled and characterized in complex two-phase micro-fluidic systems, aiming to enhance their applicability in advanced droplet-based single-cell sorting and analytical methodologies. An innovative Computational Fluid Dynamics (CFD) driven multi-dimensional optimization strategy was introduced and applied to explore the influence of geometric con-figurations and flow parameters. The droplet generation process has been studied by finite element modelling (FEM), utilizing the Laminar Flow and Level Set modules within COMSOL Multiphysics for numerical simulations. The proposed microfluidic system was manufactured and its behaviour was com-pared to the simulated characteristics. The experimental results supported the comprehension of the droplet formation in case variable volumetric flow rates and the interaction between flow dynamics and channel geometry. The effective control of the resultant droplet size distribution enables to determine the numbers of particles and cells confined.