2025 SMSI Bannerklein

OT6.97 - Electroporation Monitoring by Machine Learning and Single Cell Morphodynamic on Lab-on-Chip

Event
EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary)
Band
Lectures
Chapter
OT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
Author(s)
G. Antonelli, A. Mencattini, G. Curci, J. Filippi, M. D'Orazio, P. Casti, E. Martinelli - University of Rome Tor Vergata, Rome (Italy)
Pages
163 - 164
DOI
10.5162/EUROSENSORSXXXVI/OT6.97
ISBN
978-3-910600-03-4
Price
free

Abstract

Electroporation is a reliable, reproducible technique to induce biological cell membrane poration. Because of the biological and clinical interest in this technique, recently, many Lab-On-Chip platforms have been proposed to understand more about deep electroporation mechanisms. This led to the discovery of many electroporation side effects, such as cell contractivity and blebbing. In this work, we propose a new sensing system based on Lab-On-Chip and machine learning to correlate these side effects observed by brightfield time-lapse microscopy with electroporation efficiency.

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