OT6.79 - Development and Characterization of Microfluidic Channels for Chromatography-on-a-Chip Applications
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Lectures
- Chapter
- OT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- R. G. Rodrigues, R. Batista, V. Chu, J. P. Conde - INESC MN, Lisbon (Portugal)
- Pages
- 155 - 156
- DOI
- 10.5162/EUROSENSORSXXXVI/OT6.79
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
In this work, we designed and fabricated a microfluidic device to perform chromatographic separations. The device consists of a column filled with silica microbeads between smaller height channels working as a frit. To characterize the column efficiency, the breakthrough method was used with a solution of fluorescein isothiocyanate (FITC) in ethanol and by measuring the fluorescence signal. Several mobile phase velocities were tested to obtain retention times and plate heights.