PT6.32 - Nanozyme activity of platinum nanoparticles prepared by pulsed laser ablation
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Poster
- Chapter
- PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- S. Kurzhals, E. Melnik, R. Hainberger - Austrian Institute of Technology GmbH, Vienna (Austria), M. Fedel, L. Sajti - RHP-Technology GmbH, Wiener Neustadt (Austria)
- Pages
- 425 - 426
- DOI
- 10.5162/EUROSENSORSXXXVI/PT6.32
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
Platinum nanoparticles (Pt-NPs) were generated by pulsed laser ablation in liquid and proved to be stable in aqueous dispersion for at least six months when stabilized by small amount of sodium citrate. Their nanozyme activity was tested photometrically with tetramethylbenzidine (TMB) in the absence and presence of hydrogen peroxide (H2O2) at 22°C, which lead to specific activities (SA) of 1.9U/mg Pt-NPs and 375U/mg Pt-NPs and limits of detection (LOD) of 1.86pmol/L and 0.026pmol/L, respectively. In comparison, a SA of 289U/mg and a LOD of 36pmol/L were determined for a recombinant horseradish peroxidase in presence of H2O2 at 22°C. In particular, the lower detec-tion limit of Pt-NPs by a factor of 1384 compared to the recombinant horseradish peroxidase indicates their great potential for future bioassays.