PT6.115 - Electrochemical Biosensor Platform for Leptospirosis Diag-nosis in Urine Samples
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Poster
- Chapter
- PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- P. Suwannin, M. Sigaud, N. Zine, A. Elaissari, A. Errachid - Universite Claude Bernard Lyon, Villeurbanne (France)
- Pages
- 437 - 438
- DOI
- 10.5162/EUROSENSORSXXXVI/PT6.115
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
We developed a sensitive genosensor to detect DNA Leptospira interrogan bacteria in urine sample that is non-invasive patient sample collection. Diazonium salt was electrodeposited onto the gold working electrode, showing external carboxylic acid from the surface, and the specific Loa22-probe to leptospires was immobilized. After that, targeted DNA testing showed the EIS change in the range of 0.5× 10-5 - 0.016 pg/ml with a correlation R2 of 0.9726. The high sensitivity was indicated 0.5× 10-5 pg/ml. In addition, the developed sensor was highly specific with other urine-contaminated bacteria.