PT6.94 - Fluorescence-based point of care device for real-time rap-id detection of SARS-CoV-2
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Poster
- Chapter
- PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- T. L. Quyen, H. V. Ngoc, A. C. Vinayaka, D. D. Bang, A. Wolff, M. Dimaki, W. E. Svendsen - Technical University of Denmark, Lyngby (Denmark)
- Pages
- 433 - 434
- DOI
- 10.5162/EUROSENSORSXXXVI/PT6.94
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
We report the development of fluorescence-based point-of-care device (fPOC) for rapid, on-site and at-site detection of SARS-CoV-2 using real-time reverse transcription loop-mediated isothermal ampli-fication (RT-rLAMP). The system includes three main parts: optical setup, heating elements, and injec-tion molded cartridge with 12 reaction chambers. By integrating the RT-LAMP on the fPOC system, the fPOC device can detect the presence of SARS-CoV-2 in clinical samples within 50 min with 99% relative accuracy, 97.5% relative specificity, and 100% relative sensitivity compared to RT-qPCR.