PT6.205 - Label-free Impedimetric Immunosensor for the Detection of Fibulin 2 as a Novel Biomarker for the Diagnosis of Hypertrophic Cardiomyopathy in Human Saliva
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Poster
- Chapter
- PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- A. A. Saeed, M. N. Abbas, N. M. Nooredeen, M. M. Eissa - National Research Centre, Giza (Egypt)
- Pages
- 459 - 460
- DOI
- 10.5162/EUROSENSORSXXXVI/PT6.205
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
a novel label-free impedimetric immunosensor was developed for the detection of a recently recog-nized as a new promising cardiac biomarker, fibulin 2 (FBLN2), for the diagnosis of hypertrophic car-diomyopathy. The approach is based on immobilization of the corresponding antibody, anti-Fibulin 2 (anti-FBLN2), onto array of gold microelectrodes functionalized with 16-mercaptohexadecanoic acid (MHDA). The assembly of 16-MHDA onto the gold working microelectrodes was confirmed by cyclic voltammetry (CV), while the interaction between FBLN2 and anti-FBLN2 was monitored using electro-chemical impedance spectroscopy (EIS). Additionally, the different stages of surface modification were characterized using contact angle measurement (CAM), FTIR and Atomic Force Microscopy (AFM) in order to confirm the success of immobilization process. The analytical performance of the proposed immunosensor was evaluated and the immunosensor exhibited a high sensitivity with a good linearity in the range from 5 to 25 pg/mL with R2 of 0.976. The developed immunosensor will be used for the detection of FBLN2 in human saliva using standard addition method.