PT6.247 - Cells and Model Particles in Lateral Focusing Microfluid-ics
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Poster
- Chapter
- PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- A. Bányai, E. Farkas, I. Székács, E. Leelőssyné Tóth, R. Horváth, P. Fürjes - HUN-REN Centre for Energy Research, Budapest (Hungary)
- Pages
- 465 - 466
- DOI
- 10.5162/EUROSENSORSXXXVI/PT6.247
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
In the field of medical diagnostics, the microfluidic devices are widely applied for implementation accel-erated sample handling steps although reliable and reproducible results are to be achieved. In many cases, the medium must be pre-filtered to extract the target before analysis. In this work the aim was to gain better understanding of particle and cell behaviour in special lateral focusing systems, dedicated for size dependent separation and lateral positioning the target above the sensor zone. Fluorescent polystyrene beads in the size range of Ø = 0.5 -16.5 μm were injected into the asymmetrically curved microfluidic system, and their size dependent trajectories and lateral positions were recorded. A particle map was defined according to their lateral positions and compared to the behaviour of real cell-types (Escherichia coli, Red Blood Cell, Saccharomyces cerevisiae, HeLa), considering their morphology and size. The results highlight the importance of living cell’s morphology significantly affecting the cell move-ment in microfluidic channels compared to the rigid, spherical beads.