PT6.259 - Detection of Nucleic Acids Based on Localized Surface Plasmon Resonance

Event

EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary)

Band

Poster

Chapter

PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems

Author(s)

N. Tarpataki, D. E. Tóth, A. Bonyár - Budapest University of Technology and Economics, Budapest (Hungary)

Pages

469 - 470

DOI

10.5162/EUROSENSORSXXXVI/PT6.259

ISBN

978-3-910600-03-4

Price

free

Abstract

The main aim of this work is to test and optimize protocols for immobilizing probe-DNA and detect its complementary target-DNA strand in a microfluidic cell, as well as to provide data on the sensitivity of the sensor. For this purpose, we tested different nanostructures, namely gold nanoislands on glass substrates, created by the solid-state dewetting method and gold nanotriangles created by chemical synthesis and bonded to the substrate by subsequent surface chemistry. The sensor element, prepared on a glass slide was bonded to a PDMS-based microfluidic system. The peak shift of localized surface plasmon resonance absorption was monitored with an optical spectrometer.