PT6.120 - PCR-free detection of miRNA biomarkers for neuro-degenerative disorders
- Event
- EUROSENSORS XXXVI
2024-09-01 - 2024-09-04
Debrecen (Hungary) - Band
- Poster
- Chapter
- PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
- Author(s)
- P. Calorenni, L. M. De Plano, A. Caccamo, E. L. Sciuto, S. Oddo, S. Conoci - University of Messina, Messina (Italy)
- Pages
- 439 - 440
- DOI
- 10.5162/EUROSENSORSXXXVI/PT6.120
- ISBN
- 978-3-910600-03-4
- Price
- free
Abstract
A PCR-free strategy for microRNAs (miRNAs or miR-) fast and accurate detection is presented. The approach relies on the functionalization of a gold working electrode (WE) with an array of oligonucleo-tide probes selective for two miRNAs biomarkers that affect some intracellular processes related to human neurodegenerative disorders. The electrochemical impedance spectroscopy (EIS) analysis performed on the modified electrode allowed to directly sense the probes/miRNA duplex formation in a fast and accurate mode that is suitable for Point-of-Care (PoC) diagnostic applications.